Science

Test for simple sugars

Aim: To test a sample of food to determine whether it contains simple sugars.

Equipment: Bunsen burner, test tube tongs, Benedict's solution, Meat pattie/Bun

Method:
1. Place about 2mL of the sample in a test tube
2. Add 5 drops of the benedicts solution and mix
3. Heat, not boil, over a bunsen burner

Results: A positive test for a simple sugar result in the solution changing from a Blue colour turning to a black colour/Purple

Conclusion
Bun: Tested positive which means there were both complex plus simple sugars.
Meat pattie: Tested positive which means there were both complex plus simple sugars.

Test for complex sugar

Aim: To test a sample of food contains complex sugars.

Equipment: Testtube, Bun/Meat pattie, Iodine solution

Method:
1. Place about 2mL of the sample in a test tube

2. Add 3-5 drops of iodine and mix

Results: If complex sugars are present the iodine solution will change from a brown colour to blue or black.

Conclusion
Bun: Ther is complex sugar in the un since the water change instantly to black/blue which means it is positive for complex sugar.
Meat pattie: There is no complex sugar in the meat patties.

Test for Protein

Aim: To test if a sample of food contains protein

Equipment: Test tube, Sodium hydroxide, Copper sulfate, Meat pattie/Bun

Method:
1. Place about 2ml of the sample into a test tube and add 5 drops of sodium hydroxide.
2. Add 5 drops of copper sulfate.
3. Shake the test tube gently from side to side.

Results: If the solutions remain blue then no protein is present. If the solution changes to a purple colour then protein is present in the sample.

Conclusion
Bun: My conclusion is that there is no protein in the bun
Meat pattie: I came to the conclusion that the nutrient is not in the meat