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Thursday 1 November 2018

Preparing a microscope slide

Science 25.10.18
Preparing a microscope slide
Making a slide

1. Place your specimen in the middle of a clean slide. Ensure that the specimen is laying flat and not folded over itself.

2. Add 2-3 drops of the stain solution. Plant cells are commonly stained with iodine, whereas animal cells are community stained with methylene blue. Both of this solution stain the nucleus of each cell and your fingers and clothes be careful.

3. Holding a coverslip by its edges in your left hand, maneuver it so that the bottom edge of the coverslip makes contact with the bottom slide and the edge of the stain solution. With your right hand, support the top edge of the coverslip with a pair of tweezers.

4. Gently lower the coverslip with the tweezers, ensureing that no air bubbles are trapped underneath the coverslip.

5. Place your slide on the microscope stage and examine it under low power and magnification.






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